Scope this standard operating procedure sop is applicable to dt apparatus. Next, put a clean electrode in the neutral buffer and press the measure or calibrate button. Any nonabrasive glassware detergent may be used for hand or automatic dishwasher cleaning. The specification information should be given in the method description. Calibration of glassware georgia southern university. Responsibility ipqa production executive officer accountability head quality assurance procedure chec. Al228 suggestions for cleaning glassware 2 rinsing it is imperative that all soap, detergents and other cleaning fluids be removed from glassware before use. Specific dimensions and tolerances for applicable instruments are given in other specifications as cited throughout this specification. The output value q o,m and b are used in equation 1. The volumetric apparatus shall be manufactured from glass of suitable chemical and thermal properties, and shall be as free as possible from visible defects. The mass of water, container and temperature is measured and recorded.
Requirements of glassware calibration calibrated weighing balance. Frequently used as a heat source in the absence of flammable materials. Allow a few liters of deionised water in a beaker to equilibrate in terms of temperature by monitoring the temperature of a thermometer placed in the water. Knowing the proper use will help ensure safe laboratory practices. Calibration of volumetric glassware jonniel vince cruz college of science, pamantasan ng lungsod ng maynila received. Despite their simplicity these items are capable of giving very precise and accurate volume measurements provided they are calibrated and properly used. Darkened brown or amber actinic glass can block ultraviolet and infrared radiation. Aug 29, 2017 this serves as an instructional video showing how to calibrate and use a volumetric buret. Chemist or above of qa shall be responsible to follow the procedure mentioned in this sop. Calibration of volumetric glassware allan fraser and associates.
Sop for calibration of glassware in pharmaceuticals. Different types of glasswares glassware with size and measure oz. Calibration of volumetric glassware colorado state university. Cocktail martini, collins highball, coupette margarita, wine glass, hurricane glass, sherry port wine glass, beer mug. Lab report calibration of volumetric flask essay 2010 words. Where possible, include corrections for known systematic effects. The calibration of small volumetric laboratory glassware author. Due to some clauses this seemingly easy demand eventually turns out to be difficult to understand. This specification covers general requirements for laboratory glass volumetric apparatus of class a and b.
Typical data obtained in the calibration of a buret for the calibration below, assume that the temperature of the water is 23ec your water temperature may be different. Standard specification for laboratory glass volumetric. Calibration of glassware introduction glassware is. Pipettes and burettes are calibrated to deliver specific volumes whereas, volumetric flasks are calibrated to contain basis. Typically, the accuracy of the standard should be ten times the accuracy of the measuring device being tested. The standard input is varied within the range of measurement of the instrument to be calibrated. If the vessel is very dirty and greasy, it can be filled with a warm soap solution for 15. Calibration handbook of measuring instruments pdf calibration handbook of measuring instruments excerpt free download as pdf file.
Drain into a waste beaker until it is at, or just below, the zero mark. Become familiar with your glassware and begin calibrating it. After cleaning, rinse the glassware with running tap water. What are the different types of laboratory glassware. Glass can be blown, bent, cut, molded, and formed into many sizes and shapes, and is therefore common in chemistry, biology, and analytical laboratories. Calibration is a comparison between a known measurement the standard and the measurement using your instrument. The water should be in the same environment in which the calibration of the volumetric apparatus is to be done. This sop is applicable for calibration of laboratory glassware in quality control department at pharmaceutical company name with location. At the simplest level, calibration is a comparison between measurementsone of known magnitude or correctness made or set with one device and another measurement made in as similar a way as possible with a second device. Objectives the objective of the experiment was to calibrate a 10 ml volumetric pipette to calibrate a 25 ml volumetric pipette to calibrate a 100 ml volumetric flask to calibrate a 50 ml measuring cylinder introduction the purpose of this experiment is to study the relationship of several types of volumetric glassware and the accuracy of measuring the volumes of liquids very precisely in. Objective to lay down a procedure for calibration of disintegration test apparatus sapo.
Total volume dispensed in your notebook your notebook should contain both horizontal and vertical lines allowing you to make the graph directly on the paper like figure 32 pg 66 in the textbook. Basic laboratory glassware and equipment american chemical. Mar 31, 2015 calibration by an accredited calibration laboratory pressure gauge calibration by an accredited calibration laboratory volumetric labware burette, pipette and volumetric flask internal calibration on receipt. Precision means that a measurement using a particular tool or implement produces similar results every single time it is used. A most important requirement of the usp is the mandatory use of glass volumetric instruments with accuracy according to class a. Calibration of volumetric glassware glass apparatus used to measure the volume of a liquid or gas is called volumetric glassware. Once you have a stable reading, set the ph meter to the buffers ph value by pressing measure a second time. Glassware is found in abundance in laboratories and comes in all shapes and sizes.
This experiment is designed to introduce you to proper analytical laboratory techniques and to the methods for evaluating analytical data. Theoretical volume was determined by dividing the mass of liquid by its reference density at a. Oct, 2019 to calibrate and use a ph meter, prepare a neutral buffer with a ph of 7 and a second buffer with a ph of 4 for acidic samples or 9. Ann cutting, getty images if there was a glass class, youd graduate with honors. Lab report calibration of volumetric flask essay 2010. This set of laboratory experiments is designed to introduce you to some of the apparatus and.
The volume of glassware, calibrated at temperature tc. Volumetricglassware are containers that have been calibrated at a specifictemperature to deliver or contain very precise amounts of liquid. Instrument calibration is intended to eliminate or reduce bias in an instruments readings over a range for all continuous values. Calibration of measuring flasks or small provers using volume transfer methods sop 171 18 calibration of graduated necktype metal volumetric field standards, volumetric transfer method sop 181 19 calibration of large necktype metal provers, volume transfer method sop 191 20 control charts for calibration of neck type provers, volume. All glassware to be calibrated must be scrupulously clean before calibration. Standard specification for laboratory glass volumetric apparatus. Calibration of laboratory volumetric glassware used in. Though it has become preferable in recent years to substitute glass vessels for cheaper, more durable and less fragile plastic materials, some substances and experiments or applications still require the use of glassware. The density of water at a particular temperature is used to convert mass into volume. Measurement and calibration handbook used in thermostats, dial thermometers, and temperature compensating devices in the better pressure gages. Calibration of volumetric apparatus at specified intervals is an important task. A frosting of the glass surface viewed when dry indicates that chemical attack has occured, and recalibration may be in. U heck all glassware for damage as even small cracks, chips or scratches affect the strength of the c glassware. Some examples are pipets, volumetric flasks and burets.
Volume, weight and delivery time at calibration temperature are common parameters for calibrations. Calibration of volumetric glassware nur farah nabilah binti ahmed zhaini muhammad imanuddin bin azman maisarah binti alias summary in this experiment, the purpose of this experiment is to investigate the measurement of the actual volume contents of volumetric glassware. Calibration of volumetric glassware essay bartleby. Calibration refers to the act of evaluating and adjusting the precision and accuracy of measurement equipment. The true input value that is corrected for both bias and random errors is expressed as an uncertainty. Doc calibration of volumetric glassware procedureexperiment. Several tests were conducted to evaluate the sources of uncertainty associated with both pipette calibration systems and the accuracy and precision of each. Used for dispensing an accurate volume of a liquid. Standard practice for calibration of laboratory volumetric. On the one hand, the requirements for volumetric flasks, transfer. To lay down the procedure for calibration of laboratory glassware. At this temperature,the conversion factor from mass of water to volume, corrected for buoyancy and glass expansion, is 1. Laboratory glassware refers to a variety of equipment used in scientific work, and traditionally made of glass. The procedure is to determine the mass of liquid the glassware will hold, and to divide this mass of liquid by the density of the liquid, obtaining the corresponding volume of liquid.
This serves as an instructional video showing how to calibrate and use a volumetric buret. The procedure is to determine the mass of liquid the glassware will hold, and to divide this mass of liquid by the density of the liquid, obtaining the corresponding volume of. We know that there are uncertainties in the reading obtained from the instrument. Borosilicate glass is transparent and can withstand thermal stress. However, the procedures specified for the determination of volume of glassware can, for the most part, also be followed for the calibration of pyknometers. The calibration is done by measuring the mass of water contained in or delivered by the glassware. Sep 08, 2016 calibration procedure for burette calibration should be carried out at 252. As is common practice, clean all glassware before use. Based on the standard input and the values obtained from the instrument, one can calibrate the instrument. The test for cleanliness of glass apparatus is that on being filled with deionised. Quartz glass can withstand very high temperatures and is transparent in certain parts of the electromagnetic spectrum.
Selected procedures for volumetric calibrations 2012 ed. While some of the procedures may seem trivial and the results. Sop on calibration of glassware pharmaceutical guidance. The calibration of small volumetric laboratory glassware.
Calibration and handling of glassware class a and class b. This experiment aimed at calibrating volumetric glassware mohr pipette by comparing its volume capacity with the calculated volume of liquid delivered. Glass must conform to specifications e438 and be calibrated in accordance with practice e542. Bring in your completed assignment, and we can help you digitize it.
This page details common laboratory equipment and their appropriate use. Al228 suggestions for cleaning glassware 3 cleaning. Calibration of laboratory volumetric glassware used in titration. Why calibration of your measuring instruments is important. Take a picture with your phone, then paste the picture into a ms word document. Laboratory glassware may be made from several types of glass, each with different capabilities and used for different purposes. This is especially important with the detergents, slight traces of which will interfere with serologic and cultural reactions.
To calibrate and use a ph meter, prepare a neutral buffer with a ph of 7 and a second buffer with a ph of 4 for acidic samples or 9. For example, if you step on a scale five times in a row, a precise scale would give. Chapters 14 in harris to be prepared for the laboratory experiment. Calibration of volumetric glassware experiment is designed to help participants to learn both theories and practical skills to effectively calibrate and verify their volumetric glassware. Calibration of glassware introduction glassware is commonly. The calibration of small volumetric laboratory glassware govinfo. The test for cleanliness of glass apparatus is that on being filled with deionised water, and the water discarded an unbroken film of water remains. If you need help, see cary in elearning or visit me during my office hours. The laboratories are designed to provide space, engineering controls, and proper environmental conditions for optimal sample storage, sample handling, analysis, and calibrations, in accordance. Volumetric glassware is a class of glass vessels that are calibrated to.